You are here:
S-Butyrylthiocholine iodide
S-Butyrylthiocholine iodide (CAS 1866-16-6) is a specialized chromogenic substrate engineered for quantitative measurement of butyrylcholinesterase (BChE, pseudocholinesterase) activity in biochemical research, clinical diagnostics, and toxicology screening. This molecule integrates a butyryl thioester moiety—a selective acyl donor for BChE over acetylcholinesterase (AChE)—with a thiocholine leaving group that enables sensitive colorimetric detection via Ellman’s reagent (DTNB). Upon enzymatic hydrolysis by BChE, the released thiocholine rapidly reacts with 5,5′-dithiobis-(2-nitrobenzoic acid) to generate the yellow-colored 5-thio-2-nitrobenzoate anion (TNB⁻), measurable at 412 nm. This intermediate is particularly valuable for assessing liver function, monitoring organophosphate exposure, screening cholinesterase inhibitors for Alzheimer’s disease research, and validating enzyme kinetics in drug discovery pipelines. Manufactured under controlled conditions, it supports research programs requiring reliable, specific substrates for pseudocholinesterase activity quantification.
Property
|
Product Name
|
S-Butyrylthiocholine iodide
|
|
CAS Number
|
1866-16-6
|
|
Chinese Name
|
S-丁酰硫代胆碱碘化物
|
|
Synonyms
|
Butyrylthiocholine iodide; BTCI; S-Butyrylthiocholine iodide salt; C₉H₂₀INOS quaternary ammonium compound
|
|
Chinese Synonyms
|
丁酰硫代胆碱碘盐; 碘化 S-丁酰硫代胆碱; BTCI 底物; 丁酰胆碱酯酶底物
|
|
Molecular Formula
|
C₉H₂₀INOS
|
|
Molecular Weight
|
317.23 g/mol
|
|
Purity
|
≥ 98% (HPLC area normalization)
|
|
Product Category
|
Biochemical Reagents / Enzyme Substrates / Cholinesterase Assay Reagents
|
|
Appearance
|
White to Off-white Crystalline Powder
|
|
Melting Point
|
~140-145°C (decomposition, literature reference recommended)
|
|
Solubility
|
Highly soluble in water (>100 mg/mL); soluble in methanol; insoluble in non-polar solvents
|
|
Storage Conditions
|
Store in a cool, dry, well-ventilated area away from light and moisture. Keep container tightly closed under inert atmosphere (nitrogen) at -20°C. Protect from oxidizing agents to avoid thioester degradation.
|
|
Shelf Life
|
12-18 months under recommended storage conditions (monitor for hydrolysis)
|
|
Packaging
|
100mg/vial, 500mg/vial, 1g/bottle, or customized according to customer requirements (amber glass, sealed with PTFE liner)
|
|
Applications
|
Butyrylcholinesterase activity assay (Ellman’s method), liver function testing, organophosphate toxicity screening, cholinesterase inhibitor drug discovery, Alzheimer’s disease research, clinical diagnostic kit development
|
|
Reactivity
|
Suitable for enzymatic hydrolysis by BChE, thiocholine release quantification via DTNB reaction, kinetic parameter determination (Km, Vmax), and inhibitor IC₅₀ screening
|
|
Special Note
|
✅ BChE-Selective Substrate—butyryl thioester provides specificity over AChE. Hygroscopic & Light-Sensitive—store desiccated at -20°C in amber vials. Part of our enzyme assay reagent series.
|
